Agaricus blazei studies sponsored by NIH (National Institute of Health)CANCER CHEMOPREVENTIVE EFFECT OF AGARICUS BLAZEI MURILL Mutsuo Kozuka-a, Harukuni Tokuda-b, Teruo Mukainaka-b, Hoyoku Nishino-b, Kuo-Hsiung Lee-a a- Natural
Products Laboratory, School of Pharmacy, University of North
Carolina at Chapel Hill, Chapel Hill, NC Agaricus blazei Murill (ABM) is a mushroom (basidiomycetous fungus) originating in Brazil, and used widely as a dietary supplement called “Agaricus”. In our work, aqueous extract of the fruit body of ABM was evaluated its cancer chemopreventive effect. The extract was evaluated in vivo for anti-tumor-initiating and anti-tumor-initiating-promoting effect. For anti-tumor-promoting assay, two-stage carcinogenesis tests of ICR mouse skin caused by 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA) and by DMBA/ultraviolet light B(UVB) were used. The ABM extract was administrated with topically and orally for 20 weeks, and formation (%) of papillomas and the number of papillomas per mouse were observed. For anti-tumor-initiating activity, inhibitory effect on SENCAR mouse skin tumors induced by peroxynitrite as an initiator and TPA as a promoter was examined. Oral administration of the extract two weeks before and after tumor initiation stage resulted in a highly significant inhibition against tumor carcinogenesis. The ABM extract exhibited significant inhibition of tumor initiation and promotion steps. Both the anti-initiating and the anti-promoting assays, the extract treatment group decreased and delayed the formation of papillomas and remarkably reduced the number of papillomas per mouse compared to the control group, respectively. These results strongly suggested that the ABM extract could be promising for cancer prevention. 43rd Annual
Meeting of the American Society of Pharmacognosy and 3rd Monroe
Wall Symposium ANTITUMOR PROMOTING EFFECTS OF AGARICUS BLAZEI MURILL Mutsuo Kozuka, Masayoshi Oyama, Harukuni Tokyda, Hoyoku Nishino, Kuo-Hsiung Lee Natural Products Laboratory, School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599-7360, Department of Biochemistry, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-0841, Japan. Abstract Agaricus blazei MURILL is a mushroom (basidiomycetous fungus) originating in Brazil, and used as a dietary supplement called “Agaricus”. Anti-tumor, antiviral, blood sugar moderating and cholesterol reducing effects have been reported for this fungus. In the current work, the aqueous extract of the fungus fruit body, cultivated by Sylvan Inc. and supplied by Atlas World USA, was evaluated in three in vivo assays for anti-tumor-promoting activity. The extract had strong inhibitory effects on two-stage carcinogenesis of mouse skin caused by 7,12-dimethyl-benz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (topical and oral administration) and by DMBA/UV B (topical and oral administration). In each assay, the extract delayed the formation of papillomas and reduced the number of papillomas per mouse compared to the control group. The results suggested that Agaricus blazei extract could be promising for cancer prevention.
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